qscript ultra supermix (Quanta Biosciences)

Quanta Biosciences is a verified supplier

Quanta Biosciences manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Quanta Biosciences qscript ultra supermix
Qscript Ultra Supermix, supplied by Quanta Biosciences, used in various techniques. Bioz Stars score: 96/100, based on 164 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/qscript+ultra/qScript+Ultra+SuperMix/custom%4095217-025%4042537668
Average 96 stars, based on 164 article reviews

qscript ultra supermix - by Bioz Stars, 2026-10

96/100 stars

Images

Synthesized:

Article Title: Association Between UGT1A1 mRNA Expression and Cis-Acting Genetic Variants and Trans-Acting Transcriptional Regulators in Human Liver Samples
Article Snippet: Genomic DNA was isolated using a Qiagen DNeasy kit (Qiagen, Germantown, MD, USA); total RNA was isolated using the Direct-zol RNA Miniprep kit (Zymo Research, Irvine, CA, USA). .. Complementary DNA (cDNA) was synthesized using the qScript Ultra Flex kit (Quantabio, Beverly, MA, USA) with gene-specific primers and oligo(dT) as described previously [ ]. .. UGT1A1 expression was measured using SYBR-Green qRT-PCR on a Quantabio Q instrument with gene-specific primers ( ). β-Actin served as the internal reference control.

Article Title: Association Between UGT1A1 mRNA Expression and Cis-Acting Genetic Variants and Trans-Acting Transcriptional Regulators in Human Liver Samples
Article Snippet: Genomic DNA was isolated using a Qiagen DNeasy kit (Qiagen, Germantown, MD, USA); total RNA was isolated using the Direct-zol RNA Miniprep kit (Zymo Research, Irvine, CA, USA). .. Complementary DNA (cDNA) was synthesized using the qScript Ultra Flex kit (Quantabio, Beverly, MA, USA) with gene-specific primers and oligo(dT) as described previously [27]. .. UGT1A1 expression was measured using SYBR-Green qRT-PCR on a Quantabio Q instrument with gene-specific primers (Table S2). β-Actin served as the internal reference control.

Reverse Transcription:

Article Title: Examining the Influenza A Virus Sialic Acid Binding Preference Predictions of a Sequence‐Based Convolutional Neural Network
Article Snippet: RNA was extracted from viral stocks of A/Guinea Fowl/Hong Kong/WF10/1999(H9N2) and A/shorebird/Delaware/172/2006(H16N3) with the Mag‐Bind Viral DNA/RNA extraction kit (Omega Biotek, Norcross, GA, USA) per manufacturer instructions. .. Reverse‐transcription of RNA into cDNA was performed using the qScript Ultra Flex Kit (Quantabio, Beverly, MA, USA) per manufacturer instructions with a final concentration of 1 μM of gene‐specific forward primers designed for assembly into a pcDNA3.1(+) (Invitrogen, Carlsbad, CA, USA) vector (Table ). .. HA genes were amplified using the NEBNext Q5 Hot Start HiFi PCR Master Mix (New England BioLabs Inc., Ipswich, MA, USA) with a final concentration of 0.5 μM each of forward and reverse primers (Table ) and 4 μL of cDNA template.

Article Title: Resistance Analysis of Weekly Islatravir Plus Lenacapavir in People with HIV at 48 Weeks
Article Snippet: .. RNA was reverse transcribed into cDNA using qScript® Ultra Flex Kit (Quantabio, Beverly, MA) with an anti-sense primer specific for HIV-1 gene region p6-RT (HXB2 nucleotides 1849-3410, GenBank: K03455.1) adapted for the target participant, with an extension time of 20 min at 55°C and 20 min at 60°C. cDNA was diluted to endpoint and amplified via nested PCR using PlatinumTM SuperFi II PCR Master Mix (Invitrogen, Waltham, MA) using the following cycling conditions: 98°C for 30 s; 98°C for 10 s, 60°C for 10 s, and 72°C for 2–4 min for 35 cycles; 72°C for 5 min. Amplicons were sequenced using Illumina next-generation sequencing (Novogene). ..

Article Title: Examining the Influenza A Virus Sialic Acid Binding Preference Predictions of a Sequence-Based Convolutional Neural Network.
Article Snippet: RNA was extracted from viral stocks of A/Guinea Fowl/ Hong Kong/WF10/1999(H9N2) and A/shorebird/Delaware/ 172/2006(H16N3) with the Mag- Bind Viral DNA/RNA extraction kit (Omega Biotek, Norcross, GA, USA) per manufacturer instructions. .. Reverse- transcription of RNA into cDNA was performed using the qScript Ultra Flex Kit (Quantabio, Beverly, MA, USA) per manufacturer instructions with a final concentration of 1 μM of gene- specific forward primers designed for assembly into a pcDNA3.1(+) (Invitrogen, Carlsbad, CA, USA) vector (Table S4). .. HA genes were amplified using the NEBNext Q5 Hot Start HiFi PCR Master Mix (New England BioLabs Inc., Ipswich, MA, USA) with a final concentration of 0.5 μM each of forward and reverse primers (Table S4) and 4 μL of cDNA template.

Concentration Assay:

Amplification:

Nested PCR:

Polymerase Chain Reaction:

Next-Generation Sequencing:

Real-time Polymerase Chain Reaction:

Article Title: A severe neurodevelopmental syndrome linked to a South Asian founder variant in the UFMylation adaptor CDK5RAP3
Article Snippet: .. For qPCR, cDNA was synthesised using the qScript Ultra Flex kit (Quantabio, Beverly, MA, USA) and Oligo(dT) primers. qPCR was performed using PowerUp SYBR Green master mix on a QuantStudioTM 6 Pro Real-Time PCR system (Thermo Fisher Scientific, Waltham, MA, USA). ..

Review